Sterilization depends on contact of the sterilizing agent with all surfaces of the item being treated. The type of agent used depends upon the nature of the item to be sterilized and the amount of time required to kill the unwanted spores is critical.
The steps of steam sterilization are:
The steps involved are:
The basic steps are:
Ozone, a form of oxygen, sterilizes by oxidation, a process that destroys organic and inorganic matter. Ozone is an unstable gas, but can be easily generated from oxygen. A 6 to 12 percent concentration of ozone continuously flows through the chamber. Penetration of ozone may be controlled by vacuum in the chamber, or enhanced by adding humidity. At completion of exposure time, oxygen is allowed to flow through the chamber to purge the ozone. Cycle time may be up to 60 minutes depending on the size of the chamber or the load.